5KLN

Crystal structure of 2-aminomuconate 6-semialdehyde dehydrogenase N169A in complex with NAD+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, D, B, C)	2-aminomuconate 6-semialdehyde dehydrogenase	polymer	520	56104.0	4	UniProt (Q83V33) Pfam (PF00171)	Pseudomonas fluorescens
2	E, G, I, K (A, D, B, C)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
3	F, H, J, L (A, D, B, C)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
4	M, N, O, P (A, D, B, C)	water	water		18.0	1179	Chemie (HOH)

Sequence modifications

A, D, B, C: 1 - 500 (UniProt: Q83V33)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 169	Asn 169	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	224416.1
Non-Polymers*	Number of molecules	8
	Total formula weight	2745.7
All*	Total formula weight	227161.7

*Water molecules are not included.