5KIL

CmlA beta-hydroxylase E377D mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CmlA protein	polymer	551	62262.9	1	UniProt (F2RB80) Pfam (PF18456) Pfam (PF12706) In PDB	Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
2	A	MU-OXO-DIIRON	non-polymer		127.7	1	Chemie (FEO)
3	A	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
4	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
5		water	water		18.0	19	Chemie (HOH)

Sequence modifications

A: 0 - 532 (UniProt: F2RB80)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Asn 189	Asp 190	see remark 999
Asp 377	Glu 377	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	62262.9
Non-Polymers*	Number of molecules	3
	Total formula weight	225.8
All*	Total formula weight	62488.7

*Water molecules are not included.