5KHR
Model of human Anaphase-promoting complex/Cyclosome complex (APC15 deletion mutant) in complex with the E2 UBE2C/UBCH10 poised for ubiquitin ligation to substrate (APC/C-CDC20-substrate-UBE2C)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Anaphase-promoting complex subunit 1 | polymer | 1944 | 216725.5 | 1 | UniProt (Q9H1A4) Pfam (PF12859) Pfam (PF20518) Pfam (PF21282) Pfam (PF18122) | Homo sapiens (Human) | APC1,Cyclosome subunit 1,Mitotic checkpoint regulator,Testis-specific gene 24 protein |
| 2 | B (B) | Anaphase-promoting complex subunit 11 | polymer | 84 | 9854.6 | 1 | UniProt (Q9NYG5) Pfam (PF12861) | Homo sapiens (Human) | APC11,Cyclosome subunit 11,Hepatocellular carcinoma-associated RING finger protein |
| 3 | C, O (C, P) | Cell division cycle protein 23 homolog | polymer | 597 | 68921.0 | 2 | UniProt (Q9UJX2) Pfam (PF04049) | Homo sapiens (Human) | Anaphase-promoting complex subunit 8,APC8,Cyclosome subunit 8 |
| 4 | D (E) | Anaphase-promoting complex subunit 16 | polymer | 110 | 11678.0 | 1 | UniProt (Q96DE5) Pfam (PF17256) | Homo sapiens (Human) | APC16,Cyclosome subunit 16 |
| 5 | E, G (F, H) | Cell division cycle protein 27 homolog | polymer | 824 | 91973.1 | 2 | UniProt (P30260) Pfam (PF00515) Pfam (PF12895) | Homo sapiens (Human) | Anaphase-promoting complex subunit 3,APC3,CDC27 homolog,CDC27Hs,H-NUC |
| 6 | F, S (G, W) | Anaphase-promoting complex subunit CDC26 | polymer | 85 | 9794.0 | 2 | UniProt (Q8NHZ8) Pfam (PF10471) | Homo sapiens (Human) | Anaphase-promoting complex subunit 12,APC12,Cell division cycle protein 26 homolog |
| 7 | H (I) | Anaphase-promoting complex subunit 4 | polymer | 818 | 93207.3 | 1 | UniProt (Q9UJX5) Pfam (PF12894) Pfam (PF12896) | Homo sapiens (Human) | APC4,Cyclosome subunit 4 |
| 8 | I, J (J, K) | Cell division cycle protein 16 homolog | polymer | 620 | 71747.5 | 2 | UniProt (Q13042) Pfam (PF12895) Pfam (PF13424) | Homo sapiens (Human) | Anaphase-promoting complex subunit 6,APC6,CDC16 homolog,CDC16Hs,Cyclosome subunit 6 |
| 9 | K (L) | Anaphase-promoting complex subunit 10 | polymer | 185 | 21282.1 | 1 | UniProt (Q9UM13) Pfam (PF03256) | Homo sapiens (Human) | APC10,Cyclosome subunit 10 |
| 10 | L (M) | Anaphase-promoting complex subunit 13 | polymer | 74 | 8528.3 | 1 | UniProt (Q9BS18) Pfam (PF05839) | Homo sapiens (Human) | APC13,Cyclosome subunit 13 |
| 11 | M (N) | Anaphase-promoting complex subunit 2 | polymer | 822 | 94023.1 | 1 | UniProt (Q9UJX6) Pfam (PF00888) Pfam (PF08672) | Homo sapiens (Human) | APC2,Cyclosome subunit 2 |
| 12 | N (O) | Anaphase-promoting complex subunit 5 | polymer | 755 | 85249.8 | 1 | UniProt (Q9UJX4) Pfam (PF21371) Pfam (PF12862) | Homo sapiens (Human) | APC5,Cyclosome subunit 5 |
| 13 | P (Q) | Ubiquitin-conjugating enzyme E2 C | polymer | 190 | 20748.4 | 1 | UniProt (O00762) Pfam (PF00179) | Homo sapiens (Human) | (E3-independent) E2 ubiquitin-conjugating enzyme C,E2 ubiquitin-conjugating enzyme C,UbcH10,Ubiquitin carrier protein C,Ubiquitin-protein ligase C |
| 14 | Q (R) | Cell division cycle protein 20 homolog | polymer | 499 | 54796.5 | 1 | UniProt (Q12834) Pfam (PF12894) Pfam (PF00400) | Homo sapiens (Human) | p55CDC |
| 15 | R (S) | HSL1 peptide | polymer | 33 | 3783.1 | 1 | UniProt (by SIFTS) (P34244) | Saccharomyces cerevisiae | |
| 16 | T, U (X, Y) | Anaphase-promoting complex subunit 7 | polymer | 565 | 63204.0 | 2 | UniProt (Q9UJX3) Pfam (PF13181) Pfam (PF13432) | Homo sapiens (Human) | APC7,Cyclosome subunit 7 |
Sequence modifications
I: 1 - 808 (UniProt: Q9UJX5)
N: 1 - 822 (UniProt: Q9UJX6)
O: 1 - 755 (UniProt: Q9UJX4)
Q: 1 - 179 (UniProt: O00762)
| PDB | External Database | Details |
|---|---|---|
| Gly 809 | - | expression tag |
| Gly 810 | - | expression tag |
| Ser 811 | - | expression tag |
| Leu 812 | - | expression tag |
| Glu 813 | - | expression tag |
| Val 814 | - | expression tag |
| Leu 815 | - | expression tag |
| Phe 816 | - | expression tag |
| Gln 817 | - | expression tag |
| Gly 818 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Glu 534 | Ser 534 | engineered mutation |
| Glu 811 | Ser 811 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Glu 221 | Ser 221 | engineered mutation |
| Glu 232 | Thr 232 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 180 | - | expression tag |
| Gly 181 | - | expression tag |
| Ser 182 | - | expression tag |
| Gly 183 | - | expression tag |
| Ser 184 | - | expression tag |
| Leu 185 | - | expression tag |
| Glu 186 | - | expression tag |
| Val 187 | - | expression tag |
| Leu 188 | - | expression tag |
| Phe 189 | - | expression tag |
| Gln 190 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 21 |
| Total formula weight | 1231156.2 | |
| All* | Total formula weight | 1231156.2 |
