5KGH

X-ray structure of a glucosamine N-Acetyltransferase from Clostridium acetobutylicum, mutant Y297F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Predicted acetyltransferase	polymer	328	38362.4	2	UniProt (Q97ML2) Pfam (PF00583) In PDB	Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
2	A, B	ACETYL COENZYME *A	non-polymer		809.6	4	Chemie (ACO)
3	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
4	B	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	419	Chemie (HOH)

Sequence modifications

A, B: 1 - 320 (UniProt: Q97ML2)

PDB	External Database	Details
Phe 297	Tyr 297	engineered mutation
Gly 321	-	expression tag
Gly 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76724.8
Non-Polymers*	Number of molecules	6
	Total formula weight	3335.8
All*	Total formula weight	80060.6

*Water molecules are not included.