5KGA

X-ray structure of a glucosamine N-Acetyltransferase from Clostridium acetobutylicum, mutant D287N, in complex with N-acetylglucosamine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Predicted acetyltransferase	polymer	328	38377.4	2	UniProt (Q97ML2) Pfam (PF00583) In PDB	Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
2	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
3	A, B	COENZYME A	non-polymer		767.5	3	Chemie (COA)
4	A, B	ACETYL COENZYME *A	non-polymer		809.6	2	Chemie (ACO)
5	A, B	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	B	TETRAMETHYLAMMONIUM ION	non-polymer		74.1	1	Chemie (TMA)
8		water	water		18.0	575	Chemie (HOH)

Sequence modifications

A, B: 1 - 320 (UniProt: Q97ML2)

PDB	External Database	Details
Asn 287	Asp 287	engineered mutation
Gly 321	-	expression tag
Gly 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76754.9
Non-Polymers*	Number of molecules	11
	Total formula weight	4597.9
All*	Total formula weight	81352.8

*Water molecules are not included.