5K4M
Directed evolutionary changes in MBL super family - NDM-1 Round 10 crystal-3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Metallo-beta-lactamase type 2 | polymer | 245 | 25988.4 | 1 | UniProt (C7C422) Pfam (PF00753) | Klebsiella pneumoniae | B2 metallo-beta-lactamase,Beta-lactamase type II,Metallo-beta-lactamase NDM-1,Metallo-beta-lactamase type II,New Delhi metallo-beta-lactamase-1,NDM-1 |
| 2 | B, C (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 3 | D (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 4 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | G, I (A) | phenylphosphonic acid | non-polymer | 158.1 | 2 | Chemie (SV7) | |||
| 6 | H (A) | P-NITROPHENOL | non-polymer | 139.1 | 1 | Chemie (NPO) | |||
| 7 | J (A) | water | water | 18.0 | 187 | Chemie (HOH) |
Sequence modifications
A: 29 - 270 (UniProt: C7C422)
| PDB | External Database | Details |
|---|---|---|
| Gly 26 | - | expression tag |
| His 27 | - | expression tag |
| Met 28 | - | expression tag |
| Pro 49 | Leu 49 | engineered mutation |
| Met 88 | Val 88 | engineered mutation |
| Gly 93 | Trp 93 | engineered mutation |
| Ala 96 | Asp 96 | engineered mutation |
| Lys 103 | Asn 103 | engineered mutation |
| Arg 151 | Gln 151 | engineered mutation |
| Val 154 | Met 154 | engineered mutation |
| Thr 166 | Asn 166 | engineered mutation |
| Arg 211 | Lys 211 | engineered mutation |
| Asp 222 | Gly 222 | engineered mutation |
| Glu 223 | Asp 223 | engineered mutation |
| Val 233 | Ala 233 | engineered mutation |
| Phe 251 | Ser 251 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 25988.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 830.0 | |
| All* | Total formula weight | 26818.3 |
