5K3F

Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - His280Asn/Fluoroacetate - Cocrystallized - Single Protomer Reacted with Ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Fluoroacetate dehalogenase	polymer	306	34107.7	1	UniProt (Q6NAM1) Pfam (PF00561)	Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
2	B (B)	Fluoroacetate dehalogenase	polymer	306	34049.6	1	UniProt (Q6NAM1) Pfam (PF00561)	Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
3	C (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D, E (A, B)	water	water		18.0	715	Chemie (HOH)

Sequence modifications

A: 1 - 302 (UniProt: Q6NAM1)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag
Asn 280	His 280	engineered mutation
Gly 303	-	expression tag
Ser 304	-	expression tag

B: 1 - 302 (UniProt: Q6NAM1)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag
Asn 280	His 280	engineered mutation
Gly 303	-	expression tag
Ser 304	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	68157.3
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	68192.7

*Water molecules are not included.