5K1P
Catalytic domain of polyspecific pyrrolysyl-tRNA synthetase mutant N346A/C348A in complex with AMPPNP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pyrrolysine--tRNA ligase | polymer | 274 | 31769.4 | 1 | UniProt (Q8PWY1) Pfam (PF01409) | Methanosarcina mazei | Pyrrolysine--tRNA(Pyl) ligase,Pyrrolysyl-tRNA synthetase,PylRS |
| 2 | B (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 3 | C, D (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | E (A) | water | water | 18.0 | 273 | Chemie (HOH) |
Sequence modifications
A: 188 - 454 (UniProt: Q8PWY1)
| PDB | External Database | Details |
|---|---|---|
| Met 187 | - | initiating methionine |
| Ala 346 | Asn 346 | engineered mutation |
| Ala 348 | Cys 348 | engineered mutation |
| His 455 | - | expression tag |
| His 456 | - | expression tag |
| His 457 | - | expression tag |
| His 458 | - | expression tag |
| His 459 | - | expression tag |
| His 460 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31769.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 554.8 | |
| All* | Total formula weight | 32324.2 |
