5K18

The NatB Acetyltransferase Complex Bound To bisubstrate inhibitor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Uncharacterized protein	polymer	766	89468.2	2	UniProt (C4YFL7) Pfam (PF09797)	Candida albicans WO-1
2	B, D (B, D)	N-terminal acetyltransferase B complex subunit NAT3	polymer	188	22003.1	2	UniProt (C4YDZ9) Pfam (PF00583)	Candida albicans WO-1
3	E, F (F, E)	Bisubstrate inhibitor	polymer	12	1188.3	2		synthetic construct
4	G, H (B, D)	COENZYME A	non-polymer		767.5	2	Chemie (COA)
5	I, J, K, L (A, B, C, D)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

A, C: 1 - 745 (UniProt: C4YFL7)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Leu 269	Ser 269	cloning artifact

B, D: 1 - 188 (UniProt: C4YDZ9)

PDB	External Database	Details
Leu 81	Ser 81	cloning artifact
Leu 162	Ser 162	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	225319.4
Non-Polymers*	Number of molecules	2
	Total formula weight	1535.1
All*	Total formula weight	226854.5

*Water molecules are not included.