5JZK

The Structure of Ultra Stable Green Fluorescent Protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	249	28107.6	2	UniProt (A0A059PIQ0) Pfam (PF01353) UniProt (by SIFTS) (P42212)	Aequorea victoria (Jellyfish)
2	C, D, G, H (A, B)	NITRATE ION	non-polymer		62.0	4	Chemie (NO3)
3	E, J (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
4	F (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	I (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	K, L (A, B)	water	water		18.0	380	Chemie (HOH)

Sequence modifications

A, B: 3 - 238 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Met -10	-	initiating methionine
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 2	-	expression tag
Arg 30	Ser 30	conflict
Cro	Thr 65	chromophore
Cro	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Leu 69	Gln 69	conflict
Ser 72	Ala 72	conflict
Arg 80	Gln 80	conflict
Tyr 164	Asn 164	conflict
Val 206	Ala 206	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56215.3
Non-Polymers*	Number of molecules	8
	Total formula weight	499.7
All*	Total formula weight	56715.0

*Water molecules are not included.