5JXS

Mutant GC216/7AA of 3D polymerase from Foot-and-Mouth Disease Virus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA dependent RNA polymerase	polymer	481	54092.3	1	UniProt (P03311) Pfam (PF00680)	Foot-and-mouth disease virus (FMDV)
2	B (B)	RNA Template	polymer	8	2541.6	1		Foot-and-mouth disease virus
3	C (C)	RNA Primer	polymer	6	1906.2	1		Foot-and-mouth disease virus
4	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E, F (A, B)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A: 1 - 470 (UniProt: P03311)

PDB	External Database	Details
Ala 216	Gly 2073	engineered mutation
Ala 217	Cys 2074	engineered mutation
Ala 471	-	expression tag
Ala 472	-	expression tag
Ala 473	-	expression tag
Leu 474	-	expression tag
Glu 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	58540.1
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	58564.4

*Water molecules are not included.