5JWR

Crystal structure of foldswitch-stabilized KaiB in complex with the N-terminal CI domain of KaiC and a dimer of KaiA C-terminal domains from Thermosynechococcus elongatus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Circadian clock protein kinase KaiC	polymer	247	27738.2	2	UniProt (Q79V60) Pfam (PF06745)	Thermosynechococcus elongatus
2	B, D (B, D)	Circadian clock protein KaiB	polymer	99	10941.0	2	UniProt (Q79V61) Pfam (PF07689)	Thermosynechococcus elongatus (strain BP-1)
3	E, F, G, H (E, F, G, H)	Circadian clock protein KaiA	polymer	145	17332.8	4	UniProt (Q79V62) Pfam (PF07688)	Thermosynechococcus elongatus
4	I, J (A, C)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	K, L, M, N, O... (A, B, C, D, E...)	water	water		18.0	106	Chemie (HOH)

Sequence modifications

A, C: 17 - 247 (UniProt: Q79V60)

PDB	External Database	Details
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Lys 16	-	expression tag
Ala 41	Arg 41	engineered mutation
Ala 173	Lys 173	engineered mutation
Asp 248	-	expression tag
Tyr 249	-	expression tag
Lys 250	-	expression tag
Asp 251	-	expression tag
Asp 252	-	expression tag
Asp 253	-	expression tag
Asp 254	-	expression tag
Lys 255	-	expression tag

B, D: 1 - 99 (UniProt: Q79V61)

PDB	External Database	Details
Ala 8	Tyr 8	engineered mutation
Ala 89	Gly 89	engineered mutation
Arg 91	Asp 91	engineered mutation
Ala 94	Tyr 94	engineered mutation

E, F, G, H: 147 - 283 (UniProt: Q79V62)

PDB	External Database	Details
Asp 139	-	expression tag
Tyr 140	-	expression tag
Lys 141	-	expression tag
Asp 142	-	expression tag
Asp 143	-	expression tag
Asp 144	-	expression tag
Asp 145	-	expression tag
Lys 146	-	expression tag
Ser 272	Cys 272	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	146689.4
Non-Polymers*	Number of molecules	2
	Total formula weight	189.9
All*	Total formula weight	146879.3

*Water molecules are not included.