5JWR
Crystal structure of foldswitch-stabilized KaiB in complex with the N-terminal CI domain of KaiC and a dimer of KaiA C-terminal domains from Thermosynechococcus elongatus
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | Circadian clock protein kinase KaiC | polymer | 247 | 27738.2 | 2 | UniProt (Q79V60) Pfam (PF06745) In PDB | Thermosynechococcus elongatus | |
2 | B, D | Circadian clock protein KaiB | polymer | 99 | 10941.0 | 2 | UniProt (Q79V61) Pfam (PF07689) In PDB | Thermosynechococcus elongatus (strain BP-1) | |
3 | E, F, G, H | Circadian clock protein KaiA | polymer | 145 | 17332.8 | 4 | UniProt (Q79V62) Pfam (PF07688) In PDB | Thermosynechococcus elongatus | |
4 | A, C | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
5 | water | water | 18.0 | 106 | Chemie (HOH) |
Sequence modifications
A, C: 17 - 247 (UniProt: Q79V60)
B, D: 1 - 99 (UniProt: Q79V61)
E, F, G, H: 147 - 283 (UniProt: Q79V62)
PDB | External Database | Details |
---|---|---|
Asp 9 | - | expression tag |
Tyr 10 | - | expression tag |
Lys 11 | - | expression tag |
Asp 12 | - | expression tag |
Asp 13 | - | expression tag |
Asp 14 | - | expression tag |
Asp 15 | - | expression tag |
Lys 16 | - | expression tag |
Ala 41 | Arg 41 | engineered mutation |
Ala 173 | Lys 173 | engineered mutation |
Asp 248 | - | expression tag |
Tyr 249 | - | expression tag |
Lys 250 | - | expression tag |
Asp 251 | - | expression tag |
Asp 252 | - | expression tag |
Asp 253 | - | expression tag |
Asp 254 | - | expression tag |
Lys 255 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala 8 | Tyr 8 | engineered mutation |
Ala 89 | Gly 89 | engineered mutation |
Arg 91 | Asp 91 | engineered mutation |
Ala 94 | Tyr 94 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Asp 139 | - | expression tag |
Tyr 140 | - | expression tag |
Lys 141 | - | expression tag |
Asp 142 | - | expression tag |
Asp 143 | - | expression tag |
Asp 144 | - | expression tag |
Asp 145 | - | expression tag |
Lys 146 | - | expression tag |
Ser 272 | Cys 272 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 146689.4 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 189.9 | |
All* | Total formula weight | 146879.3 |