5JWQ

Crystal structure of KaiC S431E in complex with foldswitch-stabilized KaiB from Thermosynechococcus elongatus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Circadian clock protein kinase KaiC	polymer	526	58641.1	2	UniProt (Q79V60) Pfam (PF06745) In PDB	Thermosynechococcus elongatus (strain BP-1)
2	B, D	Circadian clock protein KaiB	polymer	99	10842.8	2	UniProt (Q79V61) Pfam (PF07689) In PDB	Thermosynechococcus elongatus (strain BP-1)
3	A, C	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

A, C: 1 - 518 (UniProt: Q79V60)

PDB	External Database	Details
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Glu 431	Ser 431	engineered mutation

B, D: 1 - 99 (UniProt: Q79V61)

PDB	External Database	Details
Ala 8	Tyr 8	engineered mutation
Ala 88	Ile 88	engineered mutation
Ala 94	Tyr 94	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	138967.7
Non-Polymers*	Number of molecules	2
	Total formula weight	854.4
All*	Total formula weight	139822.1

*Water molecules are not included.