5JWO

Crystal structure of foldswitch-stabilized KaiB in complex with the N-terminal CI domain of KaiC from Thermosynechococcus elongatus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Circadian clock protein kinase KaiC	polymer	247	27738.2	1	UniProt (Q79V60) Pfam (PF06745)	Thermosynechococcus elongatus
2	B (B)	Circadian clock protein KaiB	polymer	99	10941.0	1	UniProt (Q79V61) Pfam (PF07689)	Thermosynechococcus elongatus (strain BP-1)
3	C (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
4	D, E (A, B)	water	water		18.0	262	Chemie (HOH)

Sequence modifications

A: 17 - 247 (UniProt: Q79V60)

PDB	External Database	Details
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Lys 16	-	expression tag
Ala 41	Arg 41	engineered mutation
Ala 173	Lys 173	engineered mutation
Asp 248	-	expression tag
Tyr 249	-	expression tag
Lys 250	-	expression tag
Asp 251	-	expression tag
Asp 252	-	expression tag
Asp 253	-	expression tag
Asp 254	-	expression tag
Lys 255	-	expression tag

B: 1 - 99 (UniProt: Q79V61)

PDB	External Database	Details
Ala 8	Tyr 8	engineered mutation
Ala 89	Gly 89	engineered mutation
Arg 91	Asp 91	engineered mutation
Ala 94	Tyr 94	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	38679.2
Non-Polymers*	Number of molecules	1
	Total formula weight	427.2
All*	Total formula weight	39106.4

*Water molecules are not included.