5JSY
The 3D structure of the Ni-reconstituted U489C variant of [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough at 1.04 Angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Periplasmic [NiFeSe] hydrogenase, small subunit | polymer | 317 | 33940.9 | 1 | UniProt (Q72AS4) Pfam (PF01058) Pfam (PF14720) | Desulfovibrio vulgaris str. Hildenborough | |
| 2 | B (B) | Periplasmic [NiFeSe] hydrogenase, large subunit, selenocysteine-containing | polymer | 507 | 55958.1 | 1 | UniProt (Q72AS3) Pfam (PF00374) | Desulfovibrio vulgaris str. Hildenborough | |
| 3 | C, D, E (A) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 3 | Chemie (SF4) | |||
| 4 | F (B) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 1 | Chemie (FCO) | |||
| 5 | G (B) | NICKEL (II) ION | non-polymer | 58.7 | 1 | Chemie (NI) | |||
| 6 | H (B) | FE (II) ION | non-polymer | 55.8 | 1 | Chemie (FE2) | |||
| 7 | I (B) | HYDROSULFURIC ACID | non-polymer | 34.1 | 1 | Chemie (H2S) | |||
| 8 | J (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 9 | K, L (A, B) | water | water | 18.0 | 988 | Chemie (HOH) |
Sequence modifications
B: 12 - 510 (UniProt: Q72AS3)
| PDB | External Database | Details |
|---|---|---|
| Trp 4 | - | expression tag |
| Ser 5 | - | expression tag |
| His 6 | - | expression tag |
| Pro 7 | - | expression tag |
| Gln 8 | - | expression tag |
| Phe 9 | - | expression tag |
| Glu 10 | - | expression tag |
| Lys 11 | - | expression tag |
| Ocs 489 | Sec 489 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 89899.0 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1374.9 | |
| All* | Total formula weight | 91273.9 |
