5JQV

Crystal structure of Cytochrome P450 BM3 heme domain T269V/L272W/L322I/A406S (WIVS) variant with iron(III) deuteroporphyrin IX bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Bifunctional cytochrome P450/NADPH--P450 reductase	polymer	471	54024.4	8	UniProt (P14779)	Bacillus megaterium (strain ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / VKM B-512)	Cytochrome P450(BM-3),Cytochrome P450BM-3,Fatty acid monooxygenase,Flavocytochrome P450 BM3
2	I, J, K, L, M... (A, B, C, D, E...)	FE(III) DEUTEROPORPHYRIN IX	non-polymer		564.4	8	Chemie (FDE)
3	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	1163	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 463 (UniProt: P14779)

PDB	External Database	Details
Val 269	Thr 270	engineered mutation
Trp 272	Leu 273	engineered mutation
Ile 322	Leu 323	engineered mutation
Ser 406	Ala 407	engineered mutation
Leu 464	-	expression tag
Glu 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	432195.4
Non-Polymers*	Number of molecules	8
	Total formula weight	4515.3
All*	Total formula weight	436710.7

*Water molecules are not included.