5JQV
Crystal structure of Cytochrome P450 BM3 heme domain T269V/L272W/L322I/A406S (WIVS) variant with iron(III) deuteroporphyrin IX bound
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E... | Bifunctional cytochrome P450/NADPH--P450 reductase | polymer | 471 | 54024.4 | 8 | UniProt (P14779) Pfam (PF00067) In PDB | Bacillus megaterium (strain ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / VKM B-512) | Cytochrome P450(BM-3),Cytochrome P450BM-3,Fatty acid monooxygenase,Flavocytochrome P450 BM3 |
2 | A, B, C, D, E... | FE(III) DEUTEROPORPHYRIN IX | non-polymer | 564.4 | 8 | Chemie (FDE) | |||
3 | water | water | 18.0 | 1163 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 1 - 463 (UniProt: P14779)
PDB | External Database | Details |
---|---|---|
Val 269 | Thr 270 | engineered mutation |
Trp 272 | Leu 273 | engineered mutation |
Ile 322 | Leu 323 | engineered mutation |
Ser 406 | Ala 407 | engineered mutation |
Leu 464 | - | expression tag |
Glu 465 | - | expression tag |
His 466 | - | expression tag |
His 467 | - | expression tag |
His 468 | - | expression tag |
His 469 | - | expression tag |
His 470 | - | expression tag |
His 471 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 432195.4 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 4515.3 | |
All* | Total formula weight | 436710.7 |