5JQU

Crystal structure of Cytochrome P450 BM3 heme domain G265F/T269V/L272W/L322I/F405M/A406S (WIVS-FM) variant with iron(III) deuteroporphyrin IX bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Bifunctional cytochrome P450/NADPH--P450 reductase	polymer	471	54098.6	8	UniProt (P14779)	Bacillus megaterium (strain ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / VKM B-512)	Cytochrome P450(BM-3),Cytochrome P450BM-3,Fatty acid monooxygenase,Flavocytochrome P450 BM3
2	I, J, K, L, M... (A, B, C, D, E...)	FE(III) DEUTEROPORPHYRIN IX	non-polymer		564.4	8	Chemie (FDE)
3	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	1196	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 463 (UniProt: P14779)

PDB	External Database	Details
Phe 265	Gly 266	engineered mutation
Val 269	Thr 270	engineered mutation
Trp 272	Leu 273	engineered mutation
Ile 322	Leu 323	engineered mutation
Met 405	Phe 406	engineered mutation
Ser 406	Ala 407	engineered mutation
Leu 464	-	expression tag
Glu 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	432788.6
Non-Polymers*	Number of molecules	8
	Total formula weight	4515.3
All*	Total formula weight	437303.9

*Water molecules are not included.