5JQP

Crystal structure of ER glucosidase II heterodimeric complex consisting of catalytic subunit and the binding domain of regulatory subunit

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alpha glucosidase-like protein	polymer	951	108501.2	1	UniProt (G0SG42) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365) Pfam (PF17137)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
2	B (B)	Glucosidase 2 subunit beta-like protein	polymer	164	17753.4	1	UniProt (G0S9M2) Pfam (PF12999)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
3	C (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
4	D, E (B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	F, G (A, B)	water	water		18.0	655	Chemie (HOH)

Sequence modifications

A: 31 - 977 (UniProt: G0SG42)

PDB	External Database	Details
Gly 27	-	expression tag
Ser 28	-	expression tag
Glu 29	-	expression tag
Phe 30	-	expression tag

B: 21 - 162 (UniProt: G0S9M2)

PDB	External Database	Details
Met -1	-	expression tag
Gly 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
His 14	-	expression tag
Ile 15	-	expression tag
Glu 16	-	expression tag
Gly 17	-	expression tag
Arg 18	-	expression tag
His 19	-	expression tag
Met 20	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	126254.5
Non-Polymers*	Number of molecules	3
	Total formula weight	202.3
All*	Total formula weight	126456.8

*Water molecules are not included.