5JPM

Structure of the complex of human complement C4 with MASP-2 rebuilt using iMDFF

Replaces:  4FXG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Complement C4-A	polymer	656	71761.7	2	UniProt (P0C0L4) Pfam (PF21145) Pfam (PF01835) Pfam (PF17791) Pfam (PF17789) Pfam (PF07703)	Homo sapiens (Human)	Acidic complement C4,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
2	B, E (B, E)	Complement C4-A	polymer	767	84430.2	2	UniProt (P0C0L4) Pfam (PF01821) Pfam (PF00207) Pfam (PF07678) Pfam (PF22661)	Homo sapiens (Human)	Acidic complement C4,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
3	C, F (C, F)	Complement C4-A	polymer	291	33115.6	2	UniProt (P0C0L4) Pfam (PF07677) Pfam (PF01759)	Homo sapiens (Human)	Acidic complement C4,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
4	G, I (G, I)	Mannan-binding lectin serine protease 2	polymer	154	16773.0	2	UniProt (O00187) Pfam (PF00084)	Homo sapiens (Human)	MBL-associated serine protease 2,Mannose-binding protein-associated serine protease 2,MASP-2
5	H, J (H, J)	Mannan-binding lectin serine protease 2	polymer	242	26527.0	2	UniProt (O00187) Pfam (PF00089)	Homo sapiens (Human)	MBL-associated serine protease 2,Mannose-binding protein-associated serine protease 2,MASP-2
6	K, L, M, N, O... (K, L, M, N, O...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	6	In PDB GlyTouCan (G15407YE)

Sequence modifications

B, E: 680 - 1446 (UniProt: P0C0L4)

PDB	External Database	Details
Ser 1201	Thr 1201	variant

G, I: 291 - 444 (UniProt: O00187)

PDB	External Database	Details
His 298	Gln 298	engineered mutation
Ala 299	Pro 299	engineered mutation

H, J: 445 - 686 (UniProt: O00187)

PDB	External Database	Details
Ala 633	Ser 633	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	465214.9
Branched	Number of molecules	6
	Total formula weight	3519.3
All*	Total formula weight	468734.2

*Water molecules are not included.