5JNX

The 6.6 A cryo-EM structure of the full-length human NPC1 in complex with the cleaved glycoprotein of Ebola virus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Niemann-Pick C1 protein	polymer	1278	142272.9	1	UniProt (O15118) Pfam (PF16414) Pfam (PF22314) Pfam (PF12349) Pfam (PF02460) In PDB	Homo sapiens (Human)
2	C, E, G	Envelope glycoprotein	polymer	158	17194.5	3	UniProt (P87666) Pfam (PF01611) In PDB	Ebola virus - Zaire (1995) (ZEBOV)	GP1,2,GP
3	D, F, H	Envelope glycoprotein	polymer	130	14882.8	3	UniProt (P87666) Pfam (PF22307) In PDB	Ebola virus - Zaire (1995) (ZEBOV)	GP1,2,GP
4	B, I, K, M, N...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	7	In PDB GlyTouCan (G42666HT)
5	J	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
6	L	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G22573RC)
7	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	9	Chemie (NAG)

Sequence modifications

C, E, G: 32 - 188 (UniProt: P87666)

PDB	External Database	Details
Arg 31	-	expression tag
Val 42	Thr 42	engineered mutation

D, F, H: 509 - 632 (UniProt: P87666)

PDB	External Database	Details
His 633	-	expression tag
His 634	-	expression tag
His 635	-	expression tag
His 636	-	expression tag
His 637	-	expression tag
His 638	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	238505.0
Branched	Number of molecules	9
	Total formula weight	4306.0
Non-Polymers*	Number of molecules	9
	Total formula weight	1990.9
All*	Total formula weight	244801.9

*Water molecules are not included.