5JI2

HslU L199Q in HslUV complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	ATP-dependent protease subunit HslV	polymer	184	20223.0	4	UniProt (B7LA29) Pfam (PF00227)	Escherichia coli (strain 55989 / EAEC)	Heat shock protein HslV
2	E, F (E, F)	ATP-dependent protease ATPase subunit HslU	polymer	449	50503.6	2	UniProt (P0A6H6) Pfam (PF00004) Pfam (PF07724)	Escherichia coli O157:H7	Heat shock protein HslU,Unfoldase HslU
3	G, H, I (A, B, D)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4	J, K (E, F)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

A, B, C, D: 0 - 175 (UniProt: B7LA29)

PDB	External Database	Details
Glu 176	-	expression tag
Phe 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag

E, F: 2 - 443 (UniProt: P0A6H6)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gln 199	Leu 199	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	181899.1
Non-Polymers*	Number of molecules	5
	Total formula weight	927.3
All*	Total formula weight	182826.4

*Water molecules are not included.