5JEC

Apo-structure of humanised RadA-mutant humRadA33F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA repair and recombination protein RadA	polymer	231	25239.8	2	UniProt (O74036) Pfam (PF08423)	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
2	C, D (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
3	E, F (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	G, H (A, B)	water	water		18.0	89	Chemie (HOH)

Sequence modifications

A, B: 108 - 349 (UniProt: O74036)

PDB	External Database	Details
Met 107	-	initiating methionine
Lys 167	Ser 167	engineered mutation
Ala 168	Val 168	engineered mutation
Met 169	Ile 169	engineered mutation
Tyr 170	Trp 170	engineered mutation
Gly 175	Asn 175	engineered mutation
Leu 182	Ile 182	engineered mutation
Leu 183	Arg 183	engineered mutation
Ser 192	Asp 192	engineered mutation
Gly 193	Pro 193	engineered mutation
Ser 194	Asp 194	engineered mutation
Asp 195	Glu 195	engineered mutation
Asp 198	Lys 198	engineered mutation
Asn 199	His 199	engineered mutation
Val 200	Ile 200	engineered mutation
Ala 201	Tyr 201	engineered mutation
Tyr 202	Val 202	engineered mutation
Ser 219	Glu 219	engineered mutation
Met 221	Lys 221	engineered mutation
Met 222	Ile 222	engineered mutation
Val 223	Lys 223	engineered mutation
Tyr 232	Val 232	engineered mutation
-	Arg 288	deletion
-	Pro 289	deletion
-	Asp 290	deletion
-	Ala 291	deletion
-	Phe 292	deletion
-	Phe 293	deletion
-	Gly 294	deletion
-	Asp 295	deletion
-	Pro 296	deletion
-	Thr 297	deletion
-	Arg 298	deletion
-	Pro 299	deletion
Asn 288	Ile 300	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50479.6
Non-Polymers*	Number of molecules	4
	Total formula weight	263.0
All*	Total formula weight	50742.6

*Water molecules are not included.