5JCM

Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Os09g0567300 protein	polymer	451	48619.1	2	UniProt (Q652L6) Pfam (PF07992) Pfam (PF21791)	Oryza sativa subsp. japonica (Rice)	Putative monodehydroascorbate reductase
2	C, F (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	D, G (A, B)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
4	E, H (A, B)	ISOASCORBIC ACID	non-polymer		176.1	2	Chemie (ISD)
5	I, J (A, B)	water	water		18.0	586	Chemie (HOH)

Sequence modifications

A, B: 4 - 435 (UniProt: Q652L6)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ala -9	-	expression tag
Ser -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag
Ala 2	-	expression tag
Ser 3	-	expression tag
Phe 349	Tyr 349	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97238.1
Non-Polymers*	Number of molecules	6
	Total formula weight	3250.2
All*	Total formula weight	100488.3

*Water molecules are not included.