5JBF
4,6-alpha-glucanotransferase GTFB (D1015N mutant) from Lactobacillus reuteri 121 complexed with maltopentaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Inactive glucansucrase | polymer | 854 | 94902.1 | 2 | UniProt (Q5SBM0) Pfam (PF02324) | Lactobacillus reuteri | |
| 2 | C, F (C, F) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 828.7 | 2 | In PDB BIRD (PRD_900030) GlyTouCan (G50146AM) | alpha-maltopentaose | ||
| 3 | D, E, G, H (D, E, G, H) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 4 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | I (I) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 504.4 | 1 | In PDB BIRD (PRD_900009) GlyTouCan (G96370VA) | alpha-maltotriose | ||
| 5 | J, N (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | K, L, M, O, P (A, B) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 7 | Q, R (A, B) | water | water | 18.0 | 703 | Chemie (HOH) |
Sequence modifications
A, B: 761 - 1614 (UniProt: Q5SBM0)
| PDB | External Database | Details |
|---|---|---|
| Met 761 | Leu 761 | cloning artifact |
| Asn 1015 | Asp 1015 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 189804.1 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 3531.1 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 560.5 | |
| All* | Total formula weight | 193895.7 |
