5J77

Mutant glyceraldehyde dehydrogenase (F34M+S405N) from Thermoplasma acidophilum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	D-glyceraldehyde dehydrogenase (NADP(+))	polymer	508	56528.0	4	UniProt (Q9HK01) Pfam (PF00171)	Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)	Glyceraldehyde DH
2	E, F, G, H (A, B, C, D)	water	water		18.0	578	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 493 (UniProt: Q9HK01)

PDB	External Database	Details
Met 34	Phe 34	engineered mutation
Asn 405	Ser 405	engineered mutation
Ser 494	-	expression tag
Gly 495	-	expression tag
Arg 496	-	expression tag
Pro 497	-	expression tag
Val 498	-	expression tag
Leu 499	-	expression tag
Gly 500	-	expression tag
Ser 501	-	expression tag
Ser 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag
His 507	-	expression tag
His 508	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	226112.1
All*	Total formula weight	226112.1

*Water molecules are not included.