5IZO

Bacillus NanoRNase A (H103A) + 2 divalent cations + PO4 at the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Bifunctional oligoribonuclease and PAP phosphatase NrnA	polymer	333	37416.8	4	UniProt (O34600) Pfam (PF01368) Pfam (PF02272)	Bacillus subtilis (strain 168)	3'(2'),5'-bisphosphate nucleotidase,3'-phosphoadenosine 5'-phosphate phosphatase,PAP phosphatase,nanoRNase
2	E, F, H, I, K... (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	8	Chemie (MN)
3	G, J, M, P (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
4	Q, R, S, T (A, B, C, D)	water	water		18.0	523	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 313 (UniProt: O34600)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Ser -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Ser -1	-	expression tag
Ala 103	His 103	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	149667.2
Non-Polymers*	Number of molecules	12
	Total formula weight	819.4
All*	Total formula weight	150486.6

*Water molecules are not included.