5IYX

Crystal structure of the Arabidopsis receptor kinase HAESA in complex with the peptide hormone IDA and the co-receptor SERK1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Receptor-like protein kinase 5	polymer	658	71675.9	1	UniProt (P47735) Pfam (PF08263) Pfam (PF00560) Pfam (PF13855)	Arabidopsis thaliana (Mouse-ear cress)	Protein HAESA
2	B (B)	Protein IDA	polymer	14	1581.8	1	UniProt (Q8LAD7)	Arabidopsis thaliana (Mouse-ear cress)	Protein INFLORESCENCE DEFICIENT IN ABSCISSION
3	C (C)	Somatic embryogenesis receptor kinase 1	polymer	201	21751.4	1	UniProt (Q94AG2) Pfam (PF08263) Pfam (PF00560)	Arabidopsis thaliana (Mouse-ear cress)	AtSERK1,Somatic embryogenesis receptor-like kinase 1
4	D, E, F, G (D, E, F, G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
5	H, I, L, M (A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
6	J, K (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	N, O, P (A, B, C)	water	water		18.0	135	Chemie (HOH)

Sequence modifications

A: 20 - 620 (UniProt: P47735)

PDB	External Database	Details
Gly 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
Met 18	-	expression tag
Gly 19	-	expression tag
Leu 621	-	expression tag
Glu 622	-	expression tag
Gly 623	-	expression tag
Ser 624	-	expression tag
Glu 625	-	expression tag
Asn 626	-	expression tag
Leu 627	-	expression tag
Tyr 628	-	expression tag
Phe 629	-	expression tag
Gln 630	-	expression tag
Gly 631	-	expression tag
Ser 632	-	expression tag
Ala 633	-	expression tag
Trp 634	-	expression tag
Ser 635	-	expression tag
His 636	-	expression tag
Pro 637	-	expression tag
Gln 638	-	expression tag
Phe 639	-	expression tag
Glu 640	-	expression tag
Lys 641	-	expression tag
Gly 642	-	expression tag
Gly 643	-	expression tag
Gly 644	-	expression tag
Ser 645	-	expression tag
Gly 646	-	expression tag
Gly 647	-	expression tag
Gly 648	-	expression tag
Ser 649	-	expression tag
Gly 650	-	expression tag
Gly 651	-	expression tag
Ser 652	-	expression tag
Ala 653	-	expression tag
Trp 654	-	expression tag
Ser 655	-	expression tag
His 656	-	expression tag
Pro 657	-	expression tag
Gln 658	-	expression tag
Phe 659	-	expression tag
Glu 660	-	expression tag
Lys 661	-	expression tag
Gly 662	-	expression tag
Ala 663	-	expression tag
His 664	-	expression tag
His 665	-	expression tag
His 666	-	expression tag
His 667	-	expression tag
His 668	-	expression tag
His 669	-	expression tag
His 670	-	expression tag
His 671	-	expression tag
His 672	-	expression tag

B: 56 - 69 (UniProt: Q8LAD7)

PDB	External Database	Details
Tyr 56	Gly 56	engineered mutation

C: 24 - 213 (UniProt: Q94AG2)

PDB	External Database	Details
Gly 20	-	expression tag
Ser 21	-	expression tag
Ser 22	-	expression tag
Met 23	-	expression tag
Asp 115	Asn 115	engineered mutation
Gln 163	Asn 163	engineered mutation
Leu 214	-	expression tag
Glu 215	-	expression tag
Asn 216	-	expression tag
Leu 217	-	expression tag
Tyr 218	-	expression tag
Phe 219	-	expression tag
Gln 220	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	95009.2
Branched	Number of molecules	4
	Total formula weight	1697.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1009.0
All*	Total formula weight	97715.8

*Water molecules are not included.