5IWE

E45Q mutant of phenazine biosynthesis protein PhzF in complex with (5R,6R)-6-azaniumyl-5-ethoxycyclohexa-1,3-diene-1-carboxylate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Trans-2,3-dihydro-3-hydroxyanthranilate isomerase	polymer	298	32255.6	1	UniProt (Q51792) Pfam (PF02567)	Pseudomonas fluorescens	Phenazine/pyocyanine biosynthesis protein PhzF
2	B (A)	(5R,6R)-6-azaniumyl-5-ethoxycyclohexa-1,3-diene-1-carboxylate	non-polymer		183.2	1	Chemie (W81)
3	C, D (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4	E, F (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	G, H (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	I (A)	water	water		18.0	242	Chemie (HOH)

Sequence modifications

A: 1 - 278 (UniProt: Q51792)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gln 45	Glu 45	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32255.6
Non-Polymers*	Number of molecules	7
	Total formula weight	703.8
All*	Total formula weight	32959.3

*Water molecules are not included.