5IUF

Bacillus NanoRNase A active site mutant bound to pAp

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Bifunctional oligoribonuclease and PAP phosphatase NrnA	polymer	333	37416.8	4	UniProt (O34600) Pfam (PF01368) Pfam (PF02272) In PDB	Bacillus subtilis (strain 168)	3'(2'),5'-bisphosphate nucleotidase,3'-phosphoadenosine 5'-phosphate phosphatase,PAP phosphatase,nanoRNase
2	A, B	ADENOSINE-3'-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (A3P)
3		water	water		18.0	840	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 313 (UniProt: O34600)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Ser -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Ser -1	-	expression tag
Ala 103	His 103	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	149667.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1708.8
All*	Total formula weight	151376.0

*Water molecules are not included.