5IT7
Structure of the Kluyveromyces lactis 80S ribosome in complex with the cricket paralysis virus IRES and eEF2
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (5) | 25S ribosomal RNA | polymer | 3270 | 1056706.4 | 1 | Kluyveromyces lactis (Yeast) | ||
| 2 | B (7) | 5S ribosomal RNA | polymer | 121 | 38951.1 | 1 | GenBank (768843563) | Kluyveromyces lactis (Yeast) | |
| 3 | C (8) | 5.8S ribosomal RNA | polymer | 157 | 50293.6 | 1 | GenBank (820806853) | Kluyveromyces lactis (Yeast) | |
| 4 | D (AA) | KLLA0D16027p | polymer | 249 | 26843.8 | 1 | UniProt (Q6CQM3) Pfam (PF00181) Pfam (PF03947) | Kluyveromyces lactis (Yeast) | |
| 5 | E (BB) | 60S ribosomal protein L3 | polymer | 384 | 43422.1 | 1 | UniProt (Q6CJR7) Pfam (PF00297) | Kluyveromyces lactis (Yeast) | |
| 6 | F (CC) | KLLA0B07139p | polymer | 360 | 38993.9 | 1 | UniProt (Q6CW41) Pfam (PF14374) Pfam (PF00573) | Kluyveromyces lactis (Yeast) | |
| 7 | G (DD) | KLLA0D06941p | polymer | 295 | 33734.8 | 1 | UniProt (Q6CRR9) Pfam (PF17144) Pfam (PF14204) | Kluyveromyces lactis (Yeast) | |
| 8 | H (EE) | KLLA0B04686p | polymer | 170 | 19492.7 | 1 | UniProt (Q6CWE7) Pfam (PF01159) | Kluyveromyces lactis (Yeast) | |
| 9 | I (FF) | KLLA0D03410p | polymer | 222 | 25112.0 | 1 | UniProt (Q6CS71) Pfam (PF08079) Pfam (PF00327) | Kluyveromyces lactis (Yeast) | |
| 10 | J (GG) | KLLA0E00573p | polymer | 233 | 25954.3 | 1 | UniProt (B4UN96) Pfam (PF01248) | Kluyveromyces lactis (Yeast) | |
| 11 | K (HH) | KLLA0F04499p | polymer | 191 | 21730.1 | 1 | UniProt (Q6CLA6) Pfam (PF00347) | Kluyveromyces lactis (Yeast) | |
| 12 | L (II) | KLLA0D05643p | polymer | 216 | 24998.0 | 1 | UniProt (Q6CRX7) Pfam (PF00252) | Kluyveromyces lactis (Yeast) | |
| 13 | M (JJ) | KLLA0F08261p | polymer | 168 | 19203.1 | 1 | UniProt (Q6CKT4) Pfam (PF00281) Pfam (PF00673) | Kluyveromyces lactis (Yeast) | |
| 14 | N (LL) | 60S ribosomal protein L13 | polymer | 197 | 22442.0 | 1 | UniProt (Q875M0) Pfam (PF01294) | Kluyveromyces lactis (Yeast) | |
| 15 | O (MM) | KLLA0B13409p | polymer | 136 | 14835.4 | 1 | UniProt (Q6CVB1) Pfam (PF00467) Pfam (PF01929) | Kluyveromyces lactis (Yeast) | |
| 16 | P (NN) | Ribosomal protein L15 | polymer | 202 | 24193.9 | 1 | UniProt (Q6CJL7) Pfam (PF00827) | Kluyveromyces lactis (Yeast) | |
| 17 | Q (OO) | KLLA0F04675p | polymer | 198 | 22254.1 | 1 | UniProt (Q6CL98) Pfam (PF00572) | Kluyveromyces lactis (Yeast) | |
| 18 | R (PP) | KLLA0A06336p | polymer | 180 | 20322.0 | 1 | UniProt (Q6CXQ6) Pfam (PF00237) | Kluyveromyces lactis (Yeast) | |
| 19 | S (QQ) | KLLA0A07227p | polymer | 184 | 20479.9 | 1 | UniProt (Q6CXL8) Pfam (PF17135) | Kluyveromyces lactis (Yeast) | |
| 20 | T (RR) | KLLA0E12453p | polymer | 188 | 21688.3 | 1 | UniProt (Q6CNH9) Pfam (PF01280) | Kluyveromyces lactis (Yeast) | |
| 21 | U (SS) | 60S ribosomal protein L20 | polymer | 169 | 20046.4 | 1 | Kluyveromyces lactis (Yeast) | ||
| 22 | V (TT) | KLLA0E23651p | polymer | 158 | 17969.9 | 1 | UniProt (Q6CM20) Pfam (PF01157) | Kluyveromyces lactis (Yeast) | |
| 23 | W (UU) | KLLA0D05181p | polymer | 100 | 11419.0 | 1 | UniProt (Q6CRZ8) Pfam (PF01776) | Kluyveromyces lactis (Yeast) | |
| 24 | X (VV) | KLLA0E06997p | polymer | 132 | 13997.4 | 1 | UniProt (Q6CP75) Pfam (PF00238) | Kluyveromyces lactis (Yeast) | |
| 25 | Y (WW) | 60S ribosomal protein L24 | polymer | 62 | 7279.5 | 1 | UniProt (P38665) Pfam (PF01246) | Kluyveromyces lactis (Yeast) | Ribosomal protein L30 |
| 26 | Z (XX) | 60S ribosomal protein L25 | polymer | 121 | 13700.0 | 1 | UniProt (P48045) Pfam (PF03939) Pfam (PF00276) | Kluyveromyces lactis (Yeast) | |
| 27 | AA (YY) | KLLA0B05742p | polymer | 125 | 14127.5 | 1 | UniProt (Q6CW97) Pfam (PF16906) Pfam (PF00467) | Kluyveromyces lactis (Yeast) | |
| 28 | BA (ZZ) | KLLA0E03455p | polymer | 134 | 15398.1 | 1 | UniProt (Q6CPN9) Pfam (PF01777) | Kluyveromyces lactis (Yeast) | |
| 29 | CA (aa) | RPL28 | polymer | 147 | 16382.2 | 1 | UniProt (Q8J287) Pfam (PF00828) | Kluyveromyces lactis (Yeast) | |
| 30 | DA (bb) | KLLA0D16071p | polymer | 57 | 6500.6 | 1 | UniProt (Q6CQM1) Pfam (PF01779) | Kluyveromyces lactis (Yeast) | |
| 31 | EA (cc) | 60S ribosomal protein L30 | polymer | 97 | 10511.3 | 1 | UniProt (P38664) Pfam (PF01248) | Kluyveromyces lactis (Yeast) | L32 |
| 32 | FA (dd) | KLLA0B02937p | polymer | 106 | 12360.5 | 1 | UniProt (Q6CWM6) Pfam (PF01198) | Kluyveromyces lactis (Yeast) | |
| 33 | GA (ee) | KLLA0E06843p | polymer | 122 | 13941.4 | 1 | UniProt (Q6CP82) Pfam (PF01655) | Kluyveromyces lactis (Yeast) | |
| 34 | HA (ff) | KLLA0D07405p | polymer | 105 | 11863.7 | 1 | UniProt (Q6CRP7) Pfam (PF01247) | Kluyveromyces lactis (Yeast) | |
| 35 | IA (gg) | KLLA0C08371p | polymer | 121 | 13579.9 | 1 | UniProt (Q6CU17) Pfam (PF01199) | Kluyveromyces lactis (Yeast) | |
| 36 | JA (hh) | KLLA0F05247p | polymer | 116 | 13677.2 | 1 | UniProt (Q6CL71) Pfam (PF00831) | Kluyveromyces lactis (Yeast) | |
| 37 | KA (ii) | 60S ribosomal protein L36 | polymer | 98 | 10925.9 | 1 | UniProt (Q6CR18) Pfam (PF01158) | Kluyveromyces lactis (Yeast) | |
| 38 | LA (jj) | Ribosomal protein L37 | polymer | 85 | 9679.2 | 1 | UniProt (Q6CUW0) Pfam (PF01907) | Kluyveromyces lactis (Yeast) | |
| 39 | MA (kk) | KLLA0C18216p | polymer | 76 | 8794.4 | 1 | UniProt (Q6CSS8) Pfam (PF01781) | Kluyveromyces lactis (Yeast) | |
| 40 | NA (ll) | 60S ribosomal protein L39 | polymer | 49 | 6114.3 | 1 | UniProt (P04650) Pfam (PF00832) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | L46,YL40 |
| 41 | OA (mm) | Ubiquitin fusion protein | polymer | 51 | 5933.2 | 1 | UniProt (Q9Y854) Pfam (PF01020) | Kluyveromyces lactis (Yeast) | |
| 42 | PA (nn) | 60S ribosomal protein L41-A | polymer | 25 | 3354.2 | 1 | UniProt (P0CX86) Pfam (PF05162) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | L47,YL41 |
| 43 | QA (oo) | 60S ribosomal protein L44 | polymer | 101 | 11676.9 | 1 | UniProt (P31027) Pfam (PF00935) | Kluyveromyces lactis (Yeast) | 60S ribosomal protein L41 |
| 44 | RA (pp) | KLLA0E05941p | polymer | 87 | 9497.1 | 1 | UniProt (Q6CPC5) Pfam (PF01780) | Kluyveromyces lactis (Yeast) | |
| 45 | SA (qq) | Ribosomal protein | polymer | 217 | 24554.9 | 1 | UniProt (Q6CWR9) Pfam (PF00687) | Kluyveromyces lactis (Yeast) | |
| 46 | TA (rr) | 60S acidic ribosomal protein P0 | polymer | 195 | 21411.6 | 1 | UniProt (Q6CW89) Pfam (PF00466) Pfam (PF17777) | Kluyveromyces lactis (Yeast) | |
| 47 | UA (KK) | uL11 | polymer | 147 | 12528.4 | 1 | Kluyveromyces lactis (Yeast) | ||
| 48 | VA (A) | 40S ribosomal protein S0 | polymer | 206 | 22882.2 | 1 | UniProt (Q6CN12) Pfam (PF00318) | Kluyveromyces lactis (Yeast) | |
| 49 | WA (B) | 40S ribosomal protein S1 | polymer | 214 | 24488.3 | 1 | UniProt (Q6CWD0) Pfam (PF01015) | Kluyveromyces lactis (Yeast) | |
| 50 | XA (C) | KLLA0F09812p | polymer | 217 | 23162.9 | 1 | UniProt (Q6CKL3) Pfam (PF00333) Pfam (PF03719) | Kluyveromyces lactis (Yeast) | |
| 51 | YA (D) | KLLA0D08305p | polymer | 223 | 24830.9 | 1 | UniProt (Q6CRK7) Pfam (PF07650) Pfam (PF00189) | Kluyveromyces lactis (Yeast) | |
| 52 | ZA (E) | 40S ribosomal protein S4 | polymer | 260 | 29486.3 | 1 | UniProt (Q6CWJ2) Pfam (PF01479) Pfam (PF00900) Pfam (PF00467) Pfam (PF16121) Pfam (PF08071) | Kluyveromyces lactis (Yeast) | |
| 53 | AB (F) | KLLA0D10659p | polymer | 206 | 22912.4 | 1 | UniProt (Q6CRA3) Pfam (PF00177) | Kluyveromyces lactis (Yeast) | |
| 54 | BB (G) | 40S ribosomal protein S6 | polymer | 226 | 25811.0 | 1 | UniProt (Q6CM04) Pfam (PF01092) | Kluyveromyces lactis (Yeast) | |
| 55 | CB (H) | KLLA0C13519p | polymer | 184 | 21033.6 | 1 | UniProt (Q6CTD6) Pfam (PF01251) | Kluyveromyces lactis (Yeast) | |
| 56 | DB (I) | 40S ribosomal protein S8 | polymer | 200 | 22511.5 | 1 | UniProt (Q6CMG3) Pfam (PF01201) | Kluyveromyces lactis (Yeast) | |
| 57 | EB (J) | KLLA0E23673p | polymer | 182 | 20882.3 | 1 | UniProt (Q6CM18) Pfam (PF00163) Pfam (PF01479) | Kluyveromyces lactis (Yeast) | |
| 58 | FB (K) | KLLA0B08173p | polymer | 96 | 11423.1 | 1 | UniProt (Q6CVZ5) Pfam (PF03501) | Kluyveromyces lactis (Yeast) | |
| 59 | GB (L) | KLLA0A10483p | polymer | 155 | 17712.7 | 1 | UniProt (Q6CX80) Pfam (PF16205) Pfam (PF00366) | Kluyveromyces lactis (Yeast) | |
| 60 | HB (M) | 40S ribosomal protein S12 | polymer | 122 | 13124.9 | 1 | UniProt (Q6CLU4) Pfam (PF01248) | Kluyveromyces lactis (Yeast) | |
| 61 | IB (N) | KLLA0F18040p | polymer | 150 | 16858.7 | 1 | UniProt (Q6CJK0) Pfam (PF08069) Pfam (PF00312) | Kluyveromyces lactis (Yeast) | |
| 62 | JB (O) | 40S ribosomal protein S14 | polymer | 127 | 13458.4 | 1 | UniProt (P27069) Pfam (PF00411) | Kluyveromyces lactis (Yeast) | RP59 |
| 63 | KB (P) | KLLA0F07843p | polymer | 123 | 13969.5 | 1 | UniProt (Q6CKV4) Pfam (PF00203) | Kluyveromyces lactis (Yeast) | |
| 64 | LB (Q) | 40S ribosomal protein S16 | polymer | 141 | 15656.3 | 1 | UniProt (Q875N2) Pfam (PF00380) | Kluyveromyces lactis (Yeast) | |
| 65 | MB (R) | KLLA0B01474p | polymer | 129 | 14729.0 | 1 | UniProt (Q6CWU3) Pfam (PF00833) | Kluyveromyces lactis (Yeast) | |
| 66 | NB (S) | KLLA0B01562p | polymer | 145 | 16953.4 | 1 | UniProt (Q6CWT9) Pfam (PF00416) | Kluyveromyces lactis (Yeast) | |
| 67 | OB (T) | KLLA0A07194p | polymer | 143 | 15747.8 | 1 | UniProt (Q6CXM0) Pfam (PF01090) | Kluyveromyces lactis (Yeast) | |
| 68 | PB (U) | KLLA0F25542p | polymer | 106 | 12032.1 | 1 | UniProt (Q6CIM1) Pfam (PF00338) | Kluyveromyces lactis (Yeast) | |
| 69 | QB (V) | 40S ribosomal protein S21 | polymer | 87 | 9797.9 | 1 | UniProt (Q6CXT6) Pfam (PF01249) | Kluyveromyces lactis (Yeast) | |
| 70 | RB (W) | 40S ribosomal protein S22 | polymer | 129 | 14513.8 | 1 | UniProt (Q6CW21) Pfam (PF00410) | Kluyveromyces lactis (Yeast) | |
| 71 | SB (X) | RPS23 | polymer | 145 | 16047.9 | 1 | UniProt (Q875M3) Pfam (PF00164) | Kluyveromyces lactis (Yeast) | |
| 72 | TB (Y) | 40S ribosomal protein S24 | polymer | 134 | 15063.4 | 1 | UniProt (Q6CU44) Pfam (PF01282) | Kluyveromyces lactis (Yeast) | |
| 73 | UB (Z) | KLLA0B06182p | polymer | 70 | 7939.2 | 1 | UniProt (Q6CW78) Pfam (PF03297) | Kluyveromyces lactis (Yeast) | |
| 74 | VB (a) | KLLA0D05115p | polymer | 100 | 11454.6 | 1 | UniProt (Q6CS01) Pfam (PF01283) | Kluyveromyces lactis (Yeast) | |
| 75 | WB (b) | 40S ribosomal protein S27 | polymer | 82 | 8884.4 | 1 | UniProt (Q6CNL2) Pfam (PF01667) | Kluyveromyces lactis (Yeast) | |
| 76 | XB (c) | 40S ribosomal protein S28 | polymer | 63 | 7073.3 | 1 | UniProt (P33285) Pfam (PF01200) | Kluyveromyces lactis (Yeast) | S33 |
| 77 | YB (d) | 40S ribosomal protein S29 | polymer | 53 | 6322.1 | 1 | UniProt (Q6CPG3) Pfam (PF00253) | Kluyveromyces lactis (Yeast) | |
| 78 | ZB (e) | KLLA0C04809p | polymer | 55 | 6329.4 | 1 | UniProt (Q6CUH5) Pfam (PF04758) | Kluyveromyces lactis (Yeast) | |
| 79 | AC (f) | Ubiquitin-40S ribosomal protein S27a | polymer | 69 | 7958.4 | 1 | UniProt (P69061) Pfam (PF01599) | Kluyveromyces lactis (Yeast) | |
| 80 | BC (g) | KLLA0E12277p | polymer | 324 | 35612.7 | 1 | UniProt (Q6CNI7) Pfam (PF00400) | Kluyveromyces lactis (Yeast) | |
| 81 | CC (2) | 18S ribosomal RNA | polymer | 1798 | 579239.6 | 1 | GenBank (49642208) | Kluyveromyces lactis (Yeast) | |
| 82 | DC (4) | cricket paralysis virus IRES | polymer | 190 | 60732.8 | 1 | Cricket paralysis virus | ||
| 83 | EC (1) | Eft2p | polymer | 827 | 91592.0 | 1 | UniProt (W7R097) | Saccharomyces cerevisiae P283 | |
| 84 | AD, AE, AF, BD, BE... (2, 5, N, 1, f) | MAGNESIUM ION | non-polymer | 24.3 | 80 | Chemie (MG) | |||
| 85 | HC, IC, JC, LC, MC... (jj, mm, oo, a, b...) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 86 | NF (1) | PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER | non-polymer | 521.2 | 1 | Chemie (GCP) | |||
| 87 | OF (1) | (2S)-1-amino-N,N,N-trimethyl-1-oxobutan-2-aminium | non-polymer | 145.2 | 1 | Chemie (6EM) |
Sequence modifications
CC: 3 - 361 (UniProt: Q6CW41)
qq: 1 - 217 (UniProt: Q6CWR9)
P: 8 - 129 (UniProt: Q6CKV4)
2: 1 - 1798 (GenBank: 49642208)
1: 5 - 831 (UniProt: W7R097)
| PDB | External Database | Details |
|---|---|---|
| Arg 3 | Ile 3 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 11 | Asp 11 | conflict |
| His 12 | Asn 12 | conflict |
| Arg 152 | Lys 152 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 130 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| G 676 | U 1506854 | conflict |
| U 678 | G 1506852 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 310 | Asp 310 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 83 |
| Total formula weight | 3187105.1 | |
| Non-Polymers* | Number of molecules | 88 |
| Total formula weight | 3003.3 | |
| All* | Total formula weight | 3190108.4 |
