5ISO
STRUCTURE OF FULL LENGTH HUMAN AMPK (NON-PHOSPHORYLATED AT T-LOOP) IN COMPLEX WITH A SMALL MOLECULE ACTIVATOR, A BENZIMIDAZOLE DERIVATIVE (991)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, C) | 5'-AMP-activated protein kinase catalytic subunit alpha-2 | polymer | 552 | 62408.6 | 2 | UniProt (P54646) Pfam (PF00069) Pfam (PF21147) Pfam (PF16579) | Homo sapiens (Human) | AMPK subunit alpha-2,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase |
| 2 | B, E (B, D) | 5'-AMP-activated protein kinase subunit beta-1 | polymer | 286 | 32448.5 | 2 | UniProt (Q9Y478) Pfam (PF16561) Pfam (PF04739) | Homo sapiens (Human) | AMPKb |
| 3 | C, F (E, F) | 5'-AMP-activated protein kinase subunit gamma-1 | polymer | 331 | 37626.3 | 2 | UniProt (P54619) Pfam (PF00571) | Homo sapiens (Human) | AMPKg |
| 4 | G, L (A, C) | STAUROSPORINE | non-polymer | 466.5 | 2 | Chemie (STU) | |||
| 5 | H, M (A, C) | 5-[[6-chloranyl-5-(1-methylindol-5-yl)-1H-benzimidazol-2-yl]oxy]-2-methyl-benzoic acid | non-polymer | 431.9 | 2 | Chemie (992) | |||
| 6 | I, J, K, N, O... (E, F) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 6 | Chemie (AMP) | |||
| 7 | Q, R, S, T, U... (A, B, E, C, D...) | water | water | 18.0 | 80 | Chemie (HOH) |
Sequence modifications
B, D: 1 - 270 (UniProt: Q9Y478)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Gly -14 | - | expression tag |
| Leu -13 | - | expression tag |
| Asn -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Ile -10 | - | expression tag |
| Phe -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Gln -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Ile -4 | - | expression tag |
| Glu -3 | - | expression tag |
| Trp -2 | - | expression tag |
| His -1 | - | expression tag |
| Glu 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 264966.7 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 3880.1 | |
| All* | Total formula weight | 268846.8 |
