5IQO

Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitutions Q134E and S138E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_T4R-T6R-D13N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Protein FimG	polymer	132	13708.9	2	UniProt (P08190) In PDB	Escherichia coli (strain K12)
2	B, D	Protein FimF	polymer	15	1751.0	2	UniProt (P08189) In PDB	Escherichia coli K-12
3	A, C	COBALT (II) ION	non-polymer		58.9	5	Chemie (CO)
4	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	C	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
6		water	water		18.0	287	Chemie (HOH)

Sequence modifications

A, C: 13 - 144 (UniProt: P08190)

PDB	External Database	Details
Glu 134	Gln 157	engineered mutation
Glu 138	Ser 161	engineered mutation

B, D: 1 - 15 (UniProt: P08189)

PDB	External Database	Details
Arg 4	Thr 26	engineered mutation
Arg 6	Thr 28	engineered mutation
Asn 13	Asp 35	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	30919.6
Non-Polymers*	Number of molecules	7
	Total formula weight	595.0
All*	Total formula weight	31514.6

*Water molecules are not included.