5IQM

Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_T4R-T6R-D13N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (G, A)	Protein FimG	polymer	132	13666.8	2	UniProt (P08190)	Escherichia coli K-12
2	C, D (F, B)	Protein FimF	polymer	15	1751.0	2	UniProt (P08189)	Escherichia coli K-12
3	E, F, G, H (G, A)	COBALT (II) ION	non-polymer		58.9	4	Chemie (CO)
4	I, J, K, L (G, A, F, B)	water	water		18.0	419	Chemie (HOH)

Sequence modifications

G, A: 13 - 144 (UniProt: P08190)

PDB	External Database	Details
Glu 134	Gln 157	engineered mutation

F, B: 1 - 15 (UniProt: P08189)

PDB	External Database	Details
Arg 4	Thr 26	engineered mutation
Arg 6	Thr 28	engineered mutation
Asn 13	Asp 35	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	30835.6
Non-Polymers*	Number of molecules	4
	Total formula weight	235.7
All*	Total formula weight	31071.3

*Water molecules are not included.