5IPM
SigmaS-transcription initiation complex with 4-nt nascent RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 242 | 26899.6 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) In PDB | Escherichia coli | RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha |
| 2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB | Escherichia coli | RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta |
| 3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli | RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta' |
| 4 | E | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10118.4 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli | RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega |
| 5 | F | RNA polymerase sigma factor RpoS | polymer | 336 | 38777.6 | 1 | UniProt (P13445) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Escherichia coli | Sigma S, Sigma-38 |
| 6 | 1 | synthetic non-template strand DNA (50-MER) | polymer | 50 | 15329.8 | 1 | synthetic construct | ||
| 7 | 2 | synthetic template strand DNA (50-MER) | polymer | 50 | 15548.0 | 1 | synthetic construct | ||
| 8 | 3 | nascent RNA 4-mer | polymer | 4 | 1440.8 | 1 | synthetic construct | ||
| 9 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A, B: 1 - 235 (UniProt: P0A7Z4)
F: 1 - 330 (UniProt: P13445)
| PDB | External Database | Details |
|---|---|---|
| Ala -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 2 | Ser 2 | conflict |
| Glu 33 | Gln 33 | conflict |
| Leu 329 | Arg 329 | conflict |
| His 331 | - | expression tag |
| His 332 | - | expression tag |
| His 333 | - | expression tag |
| His 334 | - | expression tag |
| His 335 | - | expression tag |
| His 336 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 441201.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 441356.4 |
