5IIC

Crystal structure of red abalone VERL repeat 3 at 2.9 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Maltose-binding periplasmic protein,Vitelline envelope sperm lysin receptor	polymer	496	54550.7	2	UniProt (P0AEX9) UniProt (Q8WR62) Pfam (PF01547) Pfam (PF11386)	Escherichia coli	MBP,MMBP,Maltodextrin-binding protein
2	C, D (C, D)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	E, F, G, H (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)	Escherichia coli

Sequence modifications

A, B: 3970 - 4336 (UniProt: P0AEX9)

PDB	External Database	Details
Glu 3966	-	expression tag
Thr 3967	-	expression tag
Gly 3968	-	expression tag
Thr 3969	-	expression tag
Ala 4051	Asp 108	engineered mutation
Ala 4052	Lys 109	engineered mutation
Ala 4141	Glu 198	engineered mutation
Ala 4142	Asn 199	engineered mutation
His 4184	Ala 241	engineered mutation
His 4188	Lys 245	engineered mutation
Ala 4208	Lys 265	engineered mutation
Val 4281	Ala 338	engineered mutation
Val 4286	Ile 343	engineered mutation
Ala 4328	Glu 385	engineered mutation
Ala 4331	Lys 388	engineered mutation
Ala 4332	Asp 389	engineered mutation
Asn 4336	Arg 393	engineered mutation
Ala 4337	-	linker
Ala 4338	-	linker
Ala 4339	-	linker

A, B: 4340 - 4453 (UniProt: Q8WR62)

PDB	External Database	Details
Leu 4454	-	expression tag
Glu 4455	-	expression tag
His 4456	-	expression tag
His 4457	-	expression tag
His 4458	-	expression tag
His 4459	-	expression tag
His 4460	-	expression tag
His 4461	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	109101.3
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	110670.7

*Water molecules are not included.