5IHJ

Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii BIDMC57

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Maltose-binding periplasmic protein,Fimbrial protein	polymer	495	53044.6	1	UniProt (P0AEX9) UniProt (A0A140QW15) Pfam (PF01547) In PDB	Escherichia coli (strain K12)	MBP,MMBP,Maltodextrin-binding protein
2	B	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	1	In PDB BIRD (PRD_900009) GlyTouCan (G96370VA)		alpha-maltotriose
3	A	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
4	A	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	A	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
6		water	water		18.0	73	Chemie (HOH)

Sequence modifications

A: 1 - 366 (UniProt: P0AEX9)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 82	Asp 108	engineered mutation
Ala 83	Lys 109	engineered mutation
Ala 87	Asp 113	engineered mutation
Ala 88	Lys 114	engineered mutation
Ala 172	Glu 198	engineered mutation
Ala 173	Asn 199	engineered mutation
Ala 239	Lys 265	engineered mutation
Ala 359	Glu 385	engineered mutation
Ala 362	Lys 388	engineered mutation
Ala 363	Asp 389	engineered mutation
Asn 367	-	linker
Ala 368	-	linker
Ala 369	-	linker
Ala 370	-	linker
Ala 1023	-	linker

A: 1024 - 1136 (UniProt: A0A140QW15)

PDB	External Database	Details
Gly 1137	-	expression tag
Ser 1138	-	expression tag
Leu 1139	-	expression tag
Glu 1140	-	expression tag
His 1141	-	expression tag
His 1142	-	expression tag
His 1143	-	expression tag
His 1144	-	expression tag
His 1145	-	expression tag
His 1146	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	53044.6
Branched	Number of molecules	1
	Total formula weight	504.4
Non-Polymers*	Number of molecules	8
	Total formula weight	606.8
All*	Total formula weight	54155.9

*Water molecules are not included.