5IFT
STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 3-b-Galactopyranosyl glucose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Probable beta-galactosidase A | polymer | 1013 | 110627.9 | 1 | UniProt (A2QAN3) Pfam (PF01301) Pfam (PF10435) Pfam (PF13363) Pfam (PF13364) | Aspergillus niger CBS 513.88 | Lactase A |
| 2 | B (B) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G42227JK) | |||
| 3 | C (C) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB GlyTouCan (G83582BK) | |||
| 4 | D (D) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G01760ZU) | |||
| 5 | E (E) | beta-D-galactopyranose-(1-3)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB GlyTouCan (G12058PE) | |||
| 6 | F, G, H, I, J... (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 6 | Chemie (NAG) | |||
| 7 | L (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 8 | M (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 9 | N, O, P (A) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 3 | Chemie (DMS) | |||
| 10 | Q (A) | water | water | 18.0 | 604 | Chemie (HOH) |
Sequence modifications
A: 1 - 1007 (UniProt: A2QAN3)
| PDB | External Database | Details |
|---|---|---|
| Gln 298 | Glu 298 | engineered mutation |
| His 1008 | - | expression tag |
| His 1009 | - | expression tag |
| His 1010 | - | expression tag |
| His 1011 | - | expression tag |
| His 1012 | - | expression tag |
| His 1013 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 110627.9 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 3723.3 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1693.2 | |
| All* | Total formula weight | 116044.4 |
