5IE5

Crystal structure of a lactonase double mutant in complex with substrate a

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Zearalenone hydrolase	polymer	264	28801.6	3	UniProt (Q8NKB0) Pfam (PF00561)	Clonostachys rosea	lactonase
2	D, E (A, B)	(3S,7R,11E)-7,14,16-trihydroxy-3-methyl-3,4,5,6,7,8,9,10-octahydro-1H-2-benzoxacyclotetradecin-1-one	non-polymer		320.4	2	Chemie (36J)
3	F, G, H (A, B, C)	water	water		18.0	301	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 264 (UniProt: Q8NKB0)

PDB	External Database	Details
Ala 102	Ser 102	engineered mutation
His 153	Val 153	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	86404.9
Non-Polymers*	Number of molecules	2
	Total formula weight	640.8
All*	Total formula weight	87045.7

*Water molecules are not included.