5I79
Crystal structure of a beta-1,4-endoglucanase mutant from Aspergillus niger in complex with sugar
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Endo-beta-1, 4-glucanase | polymer | 304 | 33954.2 | 2 | UniProt (A0A023UH08) Pfam (PF00150) UniProt (by SIFTS) (O74706) | Aspergillus niger | |
| 2 | C, D (C, D) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 666.6 | 2 | In PDB BIRD (PRD_900011) GlyTouCan (G00025MO) | beta-cellotetraose | ||
| 3 | E, G, H (A, B) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 3 | Chemie (PGE) | |||
| 4 | F, K (A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 5 | I, J (B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | L, M (A, B) | water | water | 18.0 | 264 | Chemie (HOH) |
Sequence modifications
A, B: 31 - 331 (UniProt: A0A023UH08)
| PDB | External Database | Details |
|---|---|---|
| Glu 29 | - | expression tag |
| Phe 30 | - | expression tag |
| Ala 267 | Glu 267 | engineered mutation |
| Leu 332 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 67908.4 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1333.2 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 973.1 | |
| All* | Total formula weight | 70214.6 |
