5I5G

Crystal Structure of Transketolase mutant-R525L from Pichia Stipitis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Transketolase	polymer	697	75010.4	1	UniProt (P34736) Pfam (PF00456) Pfam (PF02779) Pfam (PF02780) In PDB	Scheffersomyces stipitis CBS 6054 (Yeast)	TK
2	A	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4		water	water		18.0	360	Chemie (HOH)

Sequence modifications

A: 1 - 677 (UniProt: P34736)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Leu 525	Arg 525	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	75010.4
Non-Polymers*	Number of molecules	2
	Total formula weight	282.1
All*	Total formula weight	75292.5

*Water molecules are not included.