5HWI

Crystal structure of selenomethionine labelled gama glutamyl cyclotransferease specific to glutathione from yeast

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glutathione-specific gamma-glutamylcyclotransferase	polymer	240	27773.6	1	UniProt (P32656) Pfam (PF04752) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Gamma-GCG
2	A	SUCCINIC ACID	non-polymer		118.1	1	Chemie (SIN)
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4		water	water		18.0	142	Chemie (HOH)

Sequence modifications

A: 1 - 232 (UniProt: P32656)

PDB	External Database	Details
Mse 42	Ser 42	engineered mutation
Met 77	Leu 77	engineered mutation
Mse 141	Ala 141	engineered mutation
Mse 176	Val 176	engineered mutation
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27773.6
Non-Polymers*	Number of molecules	2
	Total formula weight	210.2
All*	Total formula weight	27983.8

*Water molecules are not included.