5HRK
The crystal structure of AsfvPolX(H115F mutant): 1nt-gap(P) DNA2:dGTP ternary complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA polymerase beta-like protein | polymer | 178 | 20618.8 | 2 | UniProt (A0A0A1E3N6) Pfam (PF01909) Pfam (PF14791) UniProt (by SIFTS) (P42494) In PDB | African swine fever virus (ASFV) | PO174L, PolX |
2 | F, C | DNA (5'-D(*GP*TP*GP*AP*GP*TP*AP*CP*A)-3') | polymer | 9 | 2779.8 | 2 | synthetic construct | ||
3 | H, E | DNA (5'-D(*CP*GP*TP*TP*CP*TP*AP*TP*GP*TP*GP*TP*AP*CP*TP*CP*AP*C)-3') | polymer | 18 | 5457.5 | 2 | synthetic construct | ||
4 | G, D | DNA (5'-D(P*AP*TP*AP*GP*AP*AP*CP*G)-3') | polymer | 8 | 2459.7 | 2 | synthetic construct | ||
5 | A, B | 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (DGT) | |||
6 | A, B | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
7 | A, B | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
8 | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 174 (UniProt: A0A0A1E3N6)
PDB | External Database | Details |
---|---|---|
Ser -3 | - | expression tag |
Gly -2 | - | expression tag |
Gly -1 | - | expression tag |
Gly 0 | - | expression tag |
Phe 115 | His 115 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 62631.6 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 1618.4 | |
All* | Total formula weight | 64250.0 |