5HNZ

Structural basis of backwards motion in kinesin-14: plus-end directed nKn669 in the nucleotide-free state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tubulin alpha-1B chain	polymer	451	50107.2	1	UniProt (P81947) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B (B)	Tubulin beta-2B chain	polymer	445	49907.8	1	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)
3	C (K)	Protein claret segregational,Protein claret segregational,Plus-end directed kinesin-1/kinesin-14,Protein claret segregational,Protein claret segregational	polymer	371	41720.4	1	UniProt (P20480) Pfam (PF00225) UniProt (by SIFTS) (P56536)	Drosophila melanogaster (Fruit fly)
4	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
6	F (B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
7	G (B)	TAXOL	non-polymer		853.9	1	Chemie (TA1)

Sequence modifications

A: 1 - 451 (UniProt: P81947)

PDB	External Database	Details
Ser 136	Leu 136	conflict
Gly 232	Ser 232	conflict
Gly 265	Ile 265	conflict
Thr 340	Ser 340	conflict
Glu 358	Gln 358	conflict

B: 1 - 455 (UniProt: Q6B856)

PDB	External Database	Details
Ala 57	Thr 55	conflict
Val 172	Met 170	conflict
Ala 298	Ser 296	conflict
Val 318	Ile 316	conflict

K: 1 - 24 (UniProt: P20480)
K: 25 - 339 (PDB: 5HNZ)
K: 340 - 371 (UniProt: P20480)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	141735.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1844.6
All*	Total formula weight	143580.0

*Water molecules are not included.