5HNY

Structural basis of backwards motion in kinesin-14: plus-end directed nKn669 in the AMPPNP state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Tubulin alpha-1B chain	polymer	438	48638.8	1	UniProt (P81947) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (Bovine)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B	Tubulin beta-2B chain	polymer	426	47809.7	1	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (Bovine)	Beta-tubulin
3	K	Protein claret segregational, Kinesin-1/Kinesin-14, Protein claret segregational	polymer	371	41720.4	1	UniProt (P20480) Pfam (PF00225) UniProt (by SIFTS) (P56536) In PDB	Drosophila melanogaster (Fruit fly)
4	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
5	A, K	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
7	B	TAXOL	non-polymer		853.9	1	Chemie (TA1)
8	K	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)

Sequence modifications

A: 2 - 439 (UniProt: P81947)

PDB	External Database	Details
Ser 136	Leu 136	conflict
Gly 232	Ser 232	conflict
Gly 265	Ile 265	conflict
Thr 340	Ser 340	conflict
Glu 358	Gln 358	conflict

B: 2 - 437 (UniProt: Q6B856)

PDB	External Database	Details
Ala 57	Thr 55	conflict
Val 172	Met 170	conflict
Ala 298	Ser 296	conflict
Val 318	Ile 316	conflict

K: 1 - 24 (UniProt: P20480)
K: 25 - 341 (PDB: 5HNY)
K: 340 - 371 (UniProt: P20480)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	138169.0
Non-Polymers*	Number of molecules	6
	Total formula weight	2375.1
All*	Total formula weight	140544.0

*Water molecules are not included.