5HNW

Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the AMPPNP state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tubulin alpha-1B chain	polymer	450	49976.0	1	UniProt (P81947) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B (B)	Tubulin beta-2B chain	polymer	444	49776.6	1	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)
3	C (K)	Protein claret segregational,KINESIN HEAVY CHAIN ISOFORM 5C	polymer	371	41620.3	1	UniProt (P20480) Pfam (PF00225) UniProt (by SIFTS) (P56536)	Drosophila melanogaster (Fruit fly)	ncd,KIF5C,ncd
4	D, H (A, K)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	E (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
6	F (B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
7	G (B)	TAXOL	non-polymer		853.9	1	Chemie (TA1)
8	I (K)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)

Sequence modifications

A: 2 - 451 (UniProt: P81947)

PDB	External Database	Details
Ser 136	Leu 136	conflict
Gly 232	Ser 232	conflict
Gly 265	Ile 265	conflict
Thr 340	Ser 340	conflict
Glu 358	Gln 358	conflict

B: 2 - 455 (UniProt: Q6B856)

PDB	External Database	Details
Ala 57	Thr 55	conflict
Val 172	Met 170	conflict
Ala 298	Ser 296	conflict
Val 318	Ile 316	conflict

K: 1 - 24 (UniProt: P20480)
K: 25 - 334 (PDB: 5HNW)
K: 335 - 371 (UniProt: P20480)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	141372.9
Non-Polymers*	Number of molecules	6
	Total formula weight	2375.1
All*	Total formula weight	143748.0

*Water molecules are not included.