5HNN

Crystal structure of the endo-beta-1,4-glucanase (Xac0030) from Xanthomonas axonopodis pv. citri with the triple mutation His174Trp, Tyr211Ala and Lys227Arg.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Cellulase	polymer	337	37238.1	3	UniProt (Q8PRD3)	Xanthomonas axonopodis pv. citri
2	D, E, F (A, B, C)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	G, H, I (A, B, C)	water	water		18.0	581	Chemie (HOH)

Sequence modifications

A, B, C: 2 - 317 (UniProt: Q8PRD3)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Trp 174	His 214	engineered mutation
Ala 211	Tyr 251	engineered mutation
Arg 227	Lys 267	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	111714.3
Non-Polymers*	Number of molecules	3
	Total formula weight	276.3
All*	Total formula weight	111990.6

*Water molecules are not included.