5HNK
Crystal structure of T5Fen in complex intact substrate and metal ions.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | X, Y | DNA (5'-D(*AP*AP*AP*AP*GP*CP*GP*TP*AP*CP*GP*C)-3') | polymer | 12 | 3680.4 | 2 | synthetic construct | ||
| 2 | A, B | Exodeoxyribonuclease | polymer | 272 | 31275.5 | 2 | UniProt (P06229) Pfam (PF02739) Pfam (PF01367) In PDB | Escherichia phage T5 | 5'-exonuclease,T5FEN, D15 exonuclease |
| 3 | Y | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | Y | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
| 5 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | water | water | 18.0 | 224 | Chemie (HOH) |
Sequence modifications
A, B: 20 - 291 (UniProt: P06229)
| PDB | External Database | Details |
|---|---|---|
| Lys 153 | Asp 153 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 69911.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 179.8 | |
| All* | Total formula weight | 70091.6 |
