5HLT

Crystal structure of pyrene- and phenanthrene-modified DNA in complex with the BpuJ1 endonuclease binding domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Restriction endonuclease R.BpuJI	polymer	288	33766.2	2	UniProt (A3FMN7) Pfam (PF11564) In PDB	Bacillus pumilus
2	L, C	DNA (5'-D(*GP*YPY*TP*AP*CP*CP*CP*GP*TP*GP*GP*A)-3')	polymer	12	3774.6	2		synthetic construct
3	M, D	DNA (5'-D(*TP*CP*CP*AP*CP*GP*GP*GP*TP*AP*YPY*C)-3')	polymer	12	3734.5	2		synthetic construct
4		water	water		18.0	50	Chemie (HOH)

Sequence modifications

A, B: 1 - 285 (UniProt: A3FMN7)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	82550.7
All*	Total formula weight	82550.7

*Water molecules are not included.