5HJM

Crystal Structure of Pyrococcus abyssi Trm5a complexed with MTA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA (guanine(37)-N1)-methyltransferase Trm5a	polymer	352	40758.5	1	UniProt (Q9V2G1) Pfam (PF18093) Pfam (PF02475)	Pyrococcus abyssi (strain GE5 / Orsay)	M1G-methyltransferase,tRNA [GM37] methyltransferase
2	B (A)	5'-DEOXY-5'-METHYLTHIOADENOSINE	non-polymer		297.3	1	Chemie (MTA)
3	C (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
4	D (A)	HOMOSERINE LACTONE	non-polymer		101.1	1	Chemie (HSL)
5	E (A)	water	water		18.0	408	Chemie (HOH)

Sequence modifications

A: 1 - 333 (UniProt: Q9V2G1)

PDB	External Database	Details
Met -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40758.5
Non-Polymers*	Number of molecules	3
	Total formula weight	457.5
All*	Total formula weight	41216.0

*Water molecules are not included.