5HIL

Crystal structure of glycine sarcosine N-methyltransferase from Methanohalophilus portucalensis in complex with S-adenosylhomocysteine and sarcosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glycine sarcosine N-methyltransferase	polymer	284	32800.3	1	UniProt (F6KV61) Pfam (PF13649) In PDB	Methanohalophilus portucalensis FDF-1
2	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
3	A	SARCOSINE	non-polymer		89.1	1	Chemie (SAR)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5		water	water		18.0	59	Chemie (HOH)

Sequence modifications

A: 1 - 263 (UniProt: F6KV61)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32800.3
Non-Polymers*	Number of molecules	7
	Total formula weight	783.8
All*	Total formula weight	33584.1

*Water molecules are not included.